I suppose he could have done it during the touchdown run—or before, even—but the cameras first caught the poopy pants as Adam Lane ran back to the sidelines after scoring for the Gators at the beginning of the second quarter against East Carolina. This is purely speculative, but if you watch the touchdown run again, with this new knowledge in mind, it kind of looks like he's feeling something at the very end of the run. Like he is trying to hold something in, using his left leg to really plant and clench. Again, purely speculative, but take a look:
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Coaches were quick to cover up Lane's ass with a towel and he eventually got himself a new pair of pants.h/t to the Shithearts at Deadspin